Journal: The FASEB Journal

Article Title: Role of Necroptosis and Neuroinflammation in CSVD ‐Associated Cognitive Decline in db/db Mice

doi: 10.1096/fj.202500772R

Figure Lengend Snippet: Bar graph showing the mRNA expression levels of (A) RIP1, (B) RIP3, and (C) MLKL in brain tissues across each mouse group. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 for comparisons with WT‐8W mice; # p < 0.05, ## p < 0.01, and ### p < 0.001 for comparisons within db/db groups across different ages.

Article Snippet: RIP1 , bs‐5805R , Bioss , 1:500.

Techniques: Expressing

Journal: The FASEB Journal

Article Title: Role of Necroptosis and Neuroinflammation in CSVD ‐Associated Cognitive Decline in db/db Mice

doi: 10.1096/fj.202500772R

Figure Lengend Snippet: (A–C) Bar graphs showing the protein expression levels of (A) RIP1, (B) RIP3, and (C) MLKL in brain tissues of each mouse group. Significant differences are denoted as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 for inter‐group comparisons with WT‐8W mice; # p < 0.05, ## p < 0.01, and ### p < 0.001 for intra‐group comparisons with db/db‐8W mice. (D) Western blot analysis showing the expression levels of RIP1, RIP3, and MLKL proteins in the brain tissues of each group.

Article Snippet: RIP1 , bs‐5805R , Bioss , 1:500.

Techniques: Expressing, Western Blot

Journal: The FASEB Journal

Article Title: Role of Necroptosis and Neuroinflammation in CSVD ‐Associated Cognitive Decline in db/db Mice

doi: 10.1096/fj.202500772R

Figure Lengend Snippet: Immunohistochemical staining and optical density analysis of RIP1, RIP3, and MLKL in the frontal cortex of mice in each group: (A) RIP1, (B) RIP3, and (C) MLKL (* p < 0.05, ** p < 0.01, and *** p < 0.001 based on inter‐group comparison with WT‐8W mice. # p < 0.05, ## p < 0.01, and ### p < 0.001 based on intra‐group comparison with 8 week mice).

Article Snippet: RIP1 , bs‐5805R , Bioss , 1:500.

Techniques: Immunohistochemical staining, Staining, Comparison

Journal: Clinical and experimental hypertension (New York, N.Y. : 1993)

Article Title: Disruption of the caspase-1/IL-1β axis alleviates myocardial Ischemia/Reperfusion injury via improvement of mitochondrial homeostasis and reduction of Pyroptosis.

doi: 10.1080/10641963.2025.2506619

Figure Lengend Snippet: Figure 4. Caspase-1 silencing reduces the secretion of IL-1β and inhibits myocardial injury due to cardiomyocyte pyroptosis. A, Western blot of caspase-1 and IL-1β proteins in myocardial tissues of I/R mice treated with sh-caspase-1#1 or sh-caspase-1#2. B, IL-1β serum levels in I/R mice treated with sh-caspase-1 measured by ELISA. C, LDH level measurement in the serum of I/R mice treated with sh-caspase-1. D, evaluation of cardiac physical function in I/R mice treated with sh-caspase-1 by echocardiography. E, myocardial infarct size in I/R mice treated with sh-caspase-1 detected by TTC staining. F, HE staining of cardiomyocyte injury in I/R mice treated with sh-caspase-1. G, cardiomyocyte pyroptosis in I/R mice treated with sh-caspase-1 evaluated by CaV3 (live cardiomyocytes, green) and EBD (necrotic cells, red) double staining. H, Western blot of RIP1, p-RIP1, RIPK3, and p-RIPK3, MLKL, and p-MLKL proteins in myocardial tissues of I/R mice treated with sh-caspase-1. I, morphological changes of myocardial cells in ultrathin sections of myocardial tissue were observed by transmission electron microscopy. n = 10 mice for each treatment. * p < .05.

Article Snippet: After 5% skimmed milk powder blocking, the membrane was probed with primary rabbit antibodies to GAPDH (ab9485, Abcam), Caspase-1 (sc -392 736, Santa Cruz Biotechnology), IL-1β (ab234437, Abcam), RIP1 (ab300617, Abcam), p-RIP1 (53286, Cell Signaling Technology, Shanghai, China), RIPK3 (ab62344, Abcam), p-RIPK3 (ab195117, Abcam), MLKL (ab255747, Abcam), p-MLKL (ab196436, Abcam) and then with secondary antibody peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) (#111035003, Jackson ImmunoResearch).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Double Staining, Transmission Assay, Electron Microscopy

Journal: Clinical and experimental hypertension (New York, N.Y. : 1993)

Article Title: Disruption of the caspase-1/IL-1β axis alleviates myocardial Ischemia/Reperfusion injury via improvement of mitochondrial homeostasis and reduction of Pyroptosis.

doi: 10.1080/10641963.2025.2506619

Figure Lengend Snippet: Figure 5. Caspase-1 silencing suppresses H/R-induced cardiomyocyte pyroptosis in vitro. A-B, Western blot of caspase-1 protein in primary cardiomyocytes after 30 min of hypoxia and reoxygenation at 4, 6 and 8 h. C, viability of H/R-induced cardiomyocytes treated with sh-caspase-1 measured by CCK-8 assay. D, LDH release in H/ R-induced cardiomyocytes treated with sh-caspase-1. E, Representative images of pyroptosis of H/R-induced cardiomyocytes treated with sh-caspase-1 measured by PI (red)/DAPI (blue) double staining. F, PI+/DAPI+ H/R-induced cardiomyocytes treated with sh-caspase-1. G, Western blot of RIP1, p-RIP1, RIPK3, and p-RIPK3, MLKL, and p-MLKL proteins in H/R-induced cardiomyocytes treated with sh-caspase-1. Cell experiments were repeated at least three times independently. * p < .05.

Article Snippet: After 5% skimmed milk powder blocking, the membrane was probed with primary rabbit antibodies to GAPDH (ab9485, Abcam), Caspase-1 (sc -392 736, Santa Cruz Biotechnology), IL-1β (ab234437, Abcam), RIP1 (ab300617, Abcam), p-RIP1 (53286, Cell Signaling Technology, Shanghai, China), RIPK3 (ab62344, Abcam), p-RIPK3 (ab195117, Abcam), MLKL (ab255747, Abcam), p-MLKL (ab196436, Abcam) and then with secondary antibody peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) (#111035003, Jackson ImmunoResearch).

Techniques: In Vitro, Western Blot, CCK-8 Assay, Double Staining

Journal: Clinical and experimental hypertension (New York, N.Y. : 1993)

Article Title: Disruption of the caspase-1/IL-1β axis alleviates myocardial Ischemia/Reperfusion injury via improvement of mitochondrial homeostasis and reduction of Pyroptosis.

doi: 10.1080/10641963.2025.2506619

Figure Lengend Snippet: Figure 7. IL-1β participates in the cardiomyocyte pyroptosis due to mitochondrial dysfunction by caspase-1. A, immunofluorescence staining of the co-localization of caspase-1 and IL-1β in the H/R-induced cardiomyocytes. B, Western blot of IL-1β protein in H/R-induced cardiomyocytes treated with sh-caspase-1. C, IL-1β levels in the supernatant of H/R-induced cardiomyocytes treated with sh-caspase-1 measured by ELISA. D, viability of H/R-induced cardiomyocytes treated with sh-caspase-1 measured by CCK-8, E, LDH release in H/R-induced cardiomyocytes treated with sh-caspase-1. F, Representative images of pyroptosis of H/R-induced cardiomyocytes treated with sh-caspase-1 measured by PI (red)/DAPI (blue) double staining. G, PI+/DAPI+ H/R-induced cardiomyocytes treated with sh-caspase-1. H, Western blot of RIP1, p-RIP1, RIPK3, and p-RIPK3, MLKL, and p-MLKL proteins in H/R-induced cardiomyocytes treated with sh-caspase-1. Cell experiments were repeated at least three times independently. * p < .05.

Article Snippet: After 5% skimmed milk powder blocking, the membrane was probed with primary rabbit antibodies to GAPDH (ab9485, Abcam), Caspase-1 (sc -392 736, Santa Cruz Biotechnology), IL-1β (ab234437, Abcam), RIP1 (ab300617, Abcam), p-RIP1 (53286, Cell Signaling Technology, Shanghai, China), RIPK3 (ab62344, Abcam), p-RIPK3 (ab195117, Abcam), MLKL (ab255747, Abcam), p-MLKL (ab196436, Abcam) and then with secondary antibody peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) (#111035003, Jackson ImmunoResearch).

Techniques: Immunofluorescence, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Double Staining